| Sat 30-Aug-2025 |
|---|
| 15:00 | Bonnyton Thistle | 2‑1 | Girvan | |
| 15:00 | Dunbar United | 2‑4 | Cumnock | |
| 15:00 | Dundonald Bluebell | 3‑2 | Edinburgh Uni | |
| 15:00 | Glasgow University | 3‑1 | Newton Stewart | |
| 15:00 | Glenafton Athletic | 3‑0 | Dalkeith Thistle | |
| 15:00 | Golspie Sutherland | 0‑3 | Coldstream | |
| 15:00 | Hawick Royal Albert Utd | 1‑4 | Threave Rovers | |
| 15:00 | Hill Of Beath Hawthorn | 4‑2 | St Andrews United | |
| 15:00 | Invergordon | 3‑1 | Dunipace | |
| 15:00 | Jeanfield Swifts | 3‑1 | Preston Athletic | |
| 15:00 | Rutherglen Glencairn | 2‑4 | Newtongrange Star | |
| 15:00 | Sauchie Juniors | 2‑0 | Hutchison Vale | |
| 15:00 | St Cadoc's | 0‑1 | Pollok | |
| 15:00 | St. Cuthbert Wanderers | 0‑2 | Burntisland Shipyard | |
| 15:00 | Tayport | 4‑1 | Dalbeattie Star | |
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